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Culturing and Manipulation of O9-1 Neural Crest Cells
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Culturing and Manipulation of O9-1 Neural Crest Cells

Culturing and Manipulation of O9-1 Neural Crest Cells

DOI:
10.3791/58346-v

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08:32 min

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October 09, 2018

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, , ,
1Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology,University of Southern California

Chapters

  • 00:04Title
  • 00:34O9-1 Cell Culture
  • 01:00Recovery of O9-1 Cells
  • 02:59Passage of O9-1 Cells
  • 04:27Freezing of O9-1 Cells
  • 05:48Manipulation of O9-1 Cells and Differentiation of O9-1 Cells
  • 07:11Results: Yap-null Cells Fail to Differentiate into SMA-positive Smooth Muscle Cells
  • 07:49Conclusion

Summary

자동 번역

자동 번역

O9-1 is a multipotent mouse neural crest cell line. Here we describe detailed step-by-step protocols for culturing O9-1 cells, differentiating O9-1 cells into specific cell types, and genetically manipulating O9-1 cells by using siRNA-mediated knockdown or CRISPR-Cas9 genome editing.

Tags

Keywords: O9-1 Cell LineNeural Crest CellsIn VitroBasal MediaConditioned MediaBasement Membrane MatrixLIFBFGFCell CultureCell AttachmentCell DissociationTrypsin-EDTA

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