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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events
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The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation Events

DOI:
10.3791/58367-v

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06:48 min

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January 07, 2019

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, , , ,
1Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur, 2INSERM Unité 1202,Institut Pasteur, 3Institut de Biochimie et Génétique Cellulaires,Université Bordeaux Segalen, 4Unit of Technology and Service Photonic BioImaging,Institut Pasteur

Chapters

  • 00:04Title
  • 00:34Visualization of Neutrophil Activation with Reverse-Inverso-MUB40-Cy5 Peptide
  • 05:16Results: RI-MUB40 Staining Increases Over Time as More Neutrophils Become Activated
  • 06:03Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.

Tags

MUB40 PeptideNeutrophil MarkerLactoferrinNeutrophil DetectionInflammatory DiseasesAnoxic CabinetCell SeparationPercoll GradientNeutrophil IsolationWashing Buffer

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