Journal
/
생화학
/
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

DOI:
10.3791/58369-v

•

08:12 min

•

September 28, 2018

•

, ,
1Molecular Plant Biology, Department of Biochemistry,University of Turku

Chapters

  • 00:04Title
  • 00:35Preparing the Blue Native (BN) Gel
  • 03:09Thylakoid Solubilization
  • 04:19BN-PAGE
  • 05:052D-SDS-PAGE
  • 06:18Results: Protein Complex Pattern and the Composition of Thylakoid Protein Complexes Solubilized with β-DM and Digitonin
  • 07:25Conclusion

Summary

자동 번역

자동 번역

A protocol for the elucidation of plant thylakoid protein complex organization and composition with blue native polyacrylamide gel electrophoresis (BN-PAGE) and 2D-SDS-PAGE is described. The protocol is optimized for Arabidopsis thaliana, but can be used for other plant species with minor modifications.

Tags

Keywords: Thylakoid MembraneProtein ComplexesBlue Native Gel ElectrophoresisPhotosynthetic Protein ComplexesProtein Complex OrganizationGradient GelAcrylamide SolutionsPolymerizationStacking Gel

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/58369?article_type=v

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
check_url/kr/58369?article_type=v

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
check_url/kr/58369?article_type=v

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
check_url/kr/58369?article_type=v

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
check_url/kr/58369?article_type=v

Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
check_url/kr/58369?article_type=v

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
check_url/kr/58369?article_type=v

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
check_url/kr/58369?article_type=v

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
check_url/kr/58369?article_type=v

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
check_url/kr/58369?article_type=v

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Read Article