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Chapters
- 00:04TItle
- 00:35Urogenital System (UGS) Dissection
- 02:04Prostate Gross Anatomy and Individual Lobe Microdissection
- 02:51Tissue Processing for 3D Culture
- 04:41Cell Plating and Culture and Sphere Harvest
- 06:38Results: Representative Mouse Prostate Lobe Analyses
- 07:41Conclusion
Genetically engineered mice are useful models for investigating prostate cancer mechanisms. Here we present a protocol to identify and dissect prostate lobes from a mouse urogenital system, differentiate them based on histology, and isolate and culture the primary prostate cells in vitro as spheroids for downstream analyses.
