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JoVE Journal Neuroscience
Isolering af voksen rygmarven kerner for massivt Parallel Single-kernen RNA sekventering
 
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Isolering af voksen rygmarven kerner for massivt Parallel Single-kernen RNA sekventering

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Vi præsenterer her, en protokol for at hurtigt isolere høj kvalitet kerner fra frisk eller frossen væv til downstream massivt parallel RNA sekvensering. Vi medtager vaskemiddel-mekaniske og hypotonic-mekaniske væv forstyrrelser og celle lysis muligheder, som begge kan bruges til isolering af kerner.

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Neurovidenskab spørgsmål 140 kerner RNA sekventering snRNA-FF. massivt parallel rygmarven saccharose gradient
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