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Assessment of the Metabolic Profile of Primary Leukemia Cells
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Assessment of the Metabolic Profile of Primary Leukemia Cells

Assessment of the Metabolic Profile of Primary Leukemia Cells

DOI:
10.3791/58426-v

•

06:21 min

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November 21, 2018

•

,
1CLIP – Childhood Leukemia Investigation Prague, Second Faculty of Medicine, Laboratory of Molecular Genetics,Charles University, Prague

Chapters

  • 00:04Title
  • 00:28Bone Marrow Mononuclear Cell Isolation and Culture
  • 01:33Extracellular Flux Analysis Preparation
  • 02:41Cell Seeding and Sensor Cartridge Loading
  • 04:11Results: Representative Leukemic Blast Extracellular Flux Analysis
  • 05:54Conclusion

Summary

자동 번역

자동 번역

Here we present a protocol for the isolation of leukemic cells from leukemia patients bone marrow and analysis of their metabolic state. Assessment of the metabolic profile of primary leukemia cells could help to better characterize the demand of primary cells and could lead up to more personalized medicine.

Tags

Keywords: Primary Leukemia CellsMetabolic ProfileMetabolic PathwayExtracellular Flux AnalysisDensity Gradient CentrifugationMononuclear CellsCell AdhesiveExtracellular Flux AnalyzerRPMI MediumCell Counting

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