Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation, Characterization, and Differentiation…
JoVE Journal
Biology

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Biology
Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
 

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

챕터

요약
Automatic Translation

The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

Tags

Keywords: Cardiac Stem Cells CSCs Adult Mouse Heart Isolation Characterization Differentiation Cardiovascular Disease Heart Failure Cardiac Stem Cell Therapy Cost-effective Ham's Balanced Salt Solution Collagenase II Tissue Digestion Cell Suspension
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter