Journal
/
환경과학
/
Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
JoVE 신문
환경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 환경과학
Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

DOI:
10.3791/58470-v

•

08:40 min

•

February 14, 2019

•

,
1Department of Plant Biology,Michigan State University, 2Department of Chemistry,Michigan State University

Chapters

  • 00:04Title
  • 00:50Isolation of Crude Thylakoid Membranes from Spinach Leaves
  • 02:51Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
  • 04:02Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
  • 05:01Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
  • 06:07Results: Native Green Gel Electrophoresis and Band Characterization
  • 08:14Conclusion

Summary

자동 번역

자동 번역

Here we present a protocol to separate solubilized thylakoid complexes by Native Green Gel electrophoresis. Green gel bands are subsequently characterized by Time Correlated Single Photon Counting (TCSPC) and basic steps for data analysis are provided.

Tags

Thylakoid Protein ComplexesNative Green Gel ElectrophoresisTime-correlated Single Photon CountingPhotosynthesis ResearchChlorophyllSpinach LeavesTMK BufferMethanol ExtractionCentrifugationSample Preparation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Extraction and Characterization of Surfactants from Atmospheric Aerosols

Measuring and Mapping Patterns of Soil Erosion and Deposition Related to Soil Carbonate Concentrations Under Agricultural Management

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Characterization of Aquatic Biofilms with Flow Cytometry

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images

Read Article