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凝胶神经元全细胞斑块夹的急性脊髓切片的制备
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Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
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凝胶神经元全细胞斑块夹的急性脊髓切片的制备

DOI:
10.3791/58479-v

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08:30 min

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January 18, 2019

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, , , , , ,
1Department of Pain,First Affiliated Hospital of Nanchang University, 2Department of Pediatrics,First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Chapters

  • 00:04Title
  • 00:39Preparation of Micropipettes and Agar Block
  • 01:30Spinal Cord Dissection and Slicing
  • 03:46Whole-cell Patch-clamp Recordings
  • 06:57Results: Voltage-clamp Recordings in SG Neurons
  • 08:03Conclusion

Summary

자동 번역

자동 번역

在这里, 我们描述了由体外脊髓切片中的明胶 (sg) 神经元制成的全细胞膜片夹记录的基本步骤。该方法可对 sg 神经元的固有膜特性、突触传递和形态表征进行研究。

Tags

Acute Spinal Cord SlicesWhole-cell Patch-clamp RecordingSubstantia Gelatinosa NeuronsSensory TransmissionNociceptive RegulationChronic PainAgar BlockSucrose-based ACSFTranscardial PerfusionSpinal Cord ExtractionLumbosacral EnlargementMeninges RemovalPia Arachnoid MembraneVentral/dorsal RootsTransverse SlicesParasagittal SlicesVibratome

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