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JoVE Journal Neuroscience
Cryosectioning simultánea de varios cerebros de roedores
 
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Cryosectioning simultánea de varios cerebros de roedores

Article DOI: 10.3791/58513-v • 06:37 min • September 18th, 2018
September 18th, 2018 •
1,2,3, 1, 5, 1,3,4, 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

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Aquí, presentamos un protocolo de congelación y sección de tejido cerebral de varios animales como una alternativa de ahorro de tiempo a procesar solo cerebros. Esto reduce la variabilidad de coloración durante la inmunohistoquímica y reduce la proyección de imagen y tiempo cryosectioning.

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Neurociencia número 139 neurociencia histología inmunohistoquímica roedor crioprotección cryosectioning criostato neuropatología
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