/

면역학 및 감염병학

/

使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量

JoVE 신문
면역학 및 감염병학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 면역학 및 감염병학

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Please note that all translations are automatically generated. Click here for the English version.

使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量

DOI:

10.3791/58528-v

•

07:33 min

•

November 27, 2019

•

Tirthankar Mohanty, Pontus Nordenfelt

¹Department of Clinical Sciences, Division of Infection Medicine,Lund University

Chapters

00:04Title
00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
02:19Segmentation of Cells in the DNA Channel and the NET Channel
03:41Single-cell Analysis of Identifiable Properties
04:19Comparison of Cell Properties to Identify NET-forming Cells
05:23Result Outputs Demeanor
06:16Results: Analysis of NET Formation
07:07Conclusion

Summary

자동 번역

在这里，我们提出了一个协议，用于生成中性粒细胞外陷阱（NETs）和操作NETQUANT，这是一种用于定量免疫荧光图像中的NET的全自动软件选项。

Tags

Keywords: NETQUANT Automated Image Analysis Neutrophil Extracellular Traps (NETs)Immunofluorescence Imaging Image Segmentation Adaptive Segmentation DNA Channel NET Channel Sample Types Batch Processing Image Metadata Zenodo Nordonfelt Lab

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

嗜中性粒细胞胞外的陷阱：如何生成和可视化

简化人力中性粒细胞胞外陷阱（英语教师）分离和处理

方法来研究中性粒细胞脂质的改变和中性粒细胞胞外陷阱随后形成

一个简单的荧光试验犬中性粒细胞胞外陷阱里放的量化

利用共焦显微镜可视化巨噬细胞胞外陷阱

使用免疫荧光显微镜鉴定帕芬-嵌入式猫动脉血栓中中性粒细胞外陷阱

微生物和化学刺激诱导的中性粒细胞胞外陷阱的形态学和组成分析

使用改良的夹心 ELISA 定量来自中性粒细胞胞外陷阱的髓过氧化物酶 DNA 和中性粒细胞弹性蛋白酶 DNA 复合物

人和小鼠组织中性粒细胞胞外陷阱的免疫荧光成像

Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils

Read Article