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Automatisierte bildbasierte Quantifizierung von neutrophilen extrazellulären Fallen mit NETQUANT
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
DOI:

07:33 min

November 27, 2019

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Chapters

  • 00:04Title
  • 00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
  • 02:19Segmentation of Cells in the DNA Channel and the NET Channel
  • 03:41Single-cell Analysis of Identifiable Properties
  • 04:19Comparison of Cell Properties to Identify NET-forming Cells
  • 05:23Result Outputs Demeanor
  • 06:16Results: Analysis of NET Formation
  • 07:07Conclusion

Summary

자동 번역

Hier stellen wir ein Protokoll zur Erzeugung von neutrophilen extrazellulären Fallen (NETs) und zum Betrieb von NETQUANT vor, einer vollautomatischen Softwareoption zur Quantifizierung von NETs in Immunfluoreszenzbildern.

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