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可视化和跟踪活果蝇黑色素细胞卵室的内源性 mRNA
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Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
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可视化和跟踪活果蝇黑色素细胞卵室的内源性 mRNA

DOI:
10.3791/58545-v

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07:39 min

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June 04, 2019

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, , ,
1Biological Sciences Department Hunter College,City University of New York, 2Program in Molecular, Cellular, and Developmental Biology,Graduate Center, City University of New York

Chapters

  • 00:04Title
  • 00:51Molecular Beacon (MB) Nurse Cell Microinjection
  • 02:38Tracking and Colocalization Analyses and Video File Preparation
  • 05:58Results: Representative MB Visualization and Tracking
  • 07:15Conclusion

Summary

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자동 번역

在这里,我们提出了一个协议,用于可视化,检测,分析和跟踪内源性mRNA贩运在活的果蝇黑色素细胞卵室使用分子信标,旋转盘共聚焦显微镜,和开源分析软件。

Tags

MRNADrosophila MelanogasterEgg ChambersRNA LocalizationLive ImagingMolecular BeaconsMicroinjectionRNA BiologyReal-time VisualizationRNA TraffickingTherapeutic TargetsZebrafishCell CultureMicroscopyImage Analysis

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