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Immunology and Infection

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JoVE Journal Immunology and Infection
艰难梭菌(p) pppp 合成酶的纯化及体外活性测定
 
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艰难梭菌(p) pppp 合成酶的纯化及体外活性测定

Article DOI: 10.3791/58547-v • 09:53 min • November 3rd, 2018
November 3rd, 2018 •
1, 1, 1
1Department of Chemistry and Biochemistry, Old Dominion University

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本文介绍了一种纯化带组氨酸标记的热释蛋白酶的方法, 并利用放射性标记底物和产物的薄层色谱法测定了体外的酶活性。酶活性测定广泛适用于任何激酶、核苷酸循环酶或磷转移反应, 其机理包括三磷酸核苷酸水解。

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免疫学与感染 第14期 蛋白质纯化 组氨酸标签 (p) ppgpp 薄层色谱 放射性核素标记 热磷酸激酶 磷转移
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