Journal
/
/
多夏巴氏杆菌自主表达采用除抑制技术
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
DOI:

05:30 min

January 23, 2019

, , , ,

Chapters

  • 00:04Title
  • 00:43Main Culture Cultivation and Harvesting
  • 03:30Results: Representative De-Repressed Protein Expression Analysis
  • 04:32Conclusion

Summary

자동 번역

该方法首次介绍了在毕赤酵母 (komagataella phaffii)高效诱导无甲醇重组蛋白生产的可靠实验程序, 并采用了碳源调节启动子 p dc和 pdf 在小规模试验中, 在可在线监测培养参数的同时, 可采用恒定的甘油饲料。

Related Videos

Read Article