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常规 rt-pcr 和 sybr 绿色 rt-qpcr 检测罗非鱼
JoVE 신문
환경과학
Author Produced
This content is Free Access.
JoVE 신문 환경과학
Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
DOI:

10:55 min

November 10, 2018

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Chapters

  • 00:00Title
  • 01:06Sample Preparation
  • 01:43RNA Extraction
  • 06:08RNA Concentration Measurement
  • 06:35cDNA Synthesis
  • 07:09Real-time Polymerase Chain Reaction (qPCR)
  • 09:27Representative Results
  • 10:22Conclusion

Summary

자동 번역

该协议使用 rt-pcr 方法诊断罗非鱼组织中的罗非鱼病毒 (tilv)。整个方法描述了从组织解剖到总 rna 提取, 然后是 cdna 合成和检测 tilv 的常规 pcr 或定量 pcr 使用 dsdna 结合染料。

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