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Immunofluoreszenz ermöglicht Höchstauflösung Bildgebung des FtsZ Ring in Cyanobacterium Prochlorococcus
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
DOI:

10:09 min

November 06, 2018

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Chapters

  • 00:04Title
  • 00:43Sample Preparation and Fixation
  • 01:44Precoating of the Coverslip with Polystyrene Beads and Coating of Poly-L-lysine onto the Bead-coated Coverslip
  • 03:08Immobilization of Cells on the Coverslip and Permeabilization of Cyanobacterial Cells
  • 04:23Photobleaching of the Chlorophyll Pigments in a Blocking Step
  • 04:51Antibody Binding
  • 06:07Image Acquisition of STORM Data
  • 07:51Results: Super-resolution Imaging of the FtsZ Ringin in the Cyanobacterium Prochlorococcus
  • 09:42Conclusion

Summary

자동 번역

Hier beschreiben wir eine Immunofluoreszenz-Methode, um die Autofluoreszenz der Cyanobakterien zu reduzieren. Nach Immunofluoreszenz wird stochastische optische Rekonstruktion Mikroskopie verwendet, um dreidimensionale Höchstauflösung Bilder der Cyanobakterien FtsZ Ring zu erhalten.

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