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利用笼子分子胶作为可活性标记的活细胞中的贵宾的时空控制核移位
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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
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利用笼子分子胶作为可活性标记的活细胞中的贵宾的时空控制核移位

DOI:
10.3791/58631-v

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10:10 min

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January 17, 2019

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, , ,
1Department of Chemistry and Biotechnology, School of Engineering,University of Tokyo, 2Riken Center for Emergent Matter Science

Chapters

  • 00:04Title
  • 00:29Preparation of Guests with CagedGlue-R Tags
  • 02:17Preparation of Hep3B Cell Sample for Microscopic Observations
  • 02:59Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
  • 04:53Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
  • 05:48Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
  • 07:04Cell Viability Assay
  • 08:20Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
  • 09:51Conclusion

Summary

자동 번역

자동 번역

该协议描述了使用笼子分子胶标签的光触发核移位的客人在活细胞中的位置。该方法对现场选择性核靶向药物的输送具有广阔的应用前景。

Tags

Nuclear TranslocationCaged Molecular GluePhotoactivatable TagsQuantum DotDrug DeliveryHEP3B CellsFluorescent DyeEndosomes

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