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Spatio-temporelle contrôlée Translocation nucléaire des invités dans les cellules vivantes à l’aide de colles moléculaires en cage comme des balises Photoactivatable
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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
DOI:

10:10 min

January 17, 2019

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Chapters

  • 00:04Title
  • 00:29Preparation of Guests with CagedGlue-R Tags
  • 02:17Preparation of Hep3B Cell Sample for Microscopic Observations
  • 02:59Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
  • 04:53Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
  • 05:48Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
  • 07:04Cell Viability Assay
  • 08:20Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
  • 09:51Conclusion

Summary

자동 번역

Ce protocole décrit la translocation nucléaire déclenchée à la lumière des clients dans l’utilisation des balises de "cage" colle moléculaire des cellules vivantes. Cette méthode est prometteuse pour administration de médicaments sélectifs site nucléaire de ciblage.

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