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Spatiotemporally controllato traslocazione nucleare di ospiti in cellule viventi utilizzando colle molecolare in gabbia come tag fuse
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Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
DOI:

10:10 min

January 17, 2019

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Chapters

  • 00:04Title
  • 00:29Preparation of Guests with CagedGlue-R Tags
  • 02:17Preparation of Hep3B Cell Sample for Microscopic Observations
  • 02:59Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
  • 04:53Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
  • 05:48Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
  • 07:04Cell Viability Assay
  • 08:20Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
  • 09:51Conclusion

Summary

자동 번역

Questo protocollo descrive la traslocazione nucleare attivato luce di ospiti in cellule viventi utilizzando tag colla molecolare in gabbia. Questo metodo è promettente per il nucleare-targeting selettivo sito drug delivery.

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