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1School of Life Sciences,The Chinese University of Hong Kong, 2State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong, 3Key Laboratory for Regenerative Medicine, Ministry of Education,The Chinese University of Hong Kong, 4Centre for Novel Biomaterials,The Chinese University of Hong Kong
Chapters
- 00:04Title
- 01:08Preparation of Breast Non-stem Cancer Cells
- 05:08Apoptotic Induction and Detection
- 07:17Isolation of Apoptotic Cells and Apoptosis Reversal Procedure
- 08:22Measurement of Breast CSC-like Cells by Flow Cytometry
- 09:11Results: CSC-like Cells Transited from Breast Non-stem Cancer Cells During Apoptosis Reversal
- 10:29Conclusion
Aqui, apresentamos um protocolo para isolar células de câncer de mama apoptotic classificando fluorescência-ativado da pilha e detectar ainda mais a transição de células-tronco de câncer de mama para células de células-tronco, como câncer de mama após a reversão de apoptose por citometria de fluxo.
