Journal
/
/
建立与艾滋病毒相关的肝脏发病机制的双人性化TK-NOG小鼠模型
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
DOI:

00:10 min

September 11, 2019

, , , ,

Chapters

  • 00:04Title
  • 00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
  • 04:25Preparation of Human Hepatocytes for Transplantation
  • 05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
  • 06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
  • 07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
  • 09:25Conclusion

Summary

자동 번역

该协议提供了一种可靠的方法,建立人类免疫系统和肝细胞的人性化小鼠。通过注射人肝细胞和CD34实现的双重组免疫缺陷小鼠造血干细胞易受人体免疫缺陷病毒-1感染和重述肝损伤的影响,如艾滋病毒感染者。

Related Videos

Read Article