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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

DOI:
10.3791/58647-v

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07:13 min

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January 07, 2019

•

, ,
1Department of Pharmacology, Faculty of Dentistry,Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health

Chapters

  • 00:04Title
  • 00:45Blood Sample Preparation and Deoxygenation
  • 02:52Red Blood Cell (RBC) Nitrite Reduction and Vasodilator-stimulated Phosphoprotein (VASP) Western Blotting
  • 04:39Results: Representative Platelet-based Nitric Oxide (NO) Detection
  • 06:18Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

Tags

PlateletNitric OxideVASP PhosphorylationBloodIn VitroIn VivoP-VASPSensorCentrifugationPlatelet-rich PlasmaTyrode BufferRed Blood CellsDeoxygenationNitrite Reduction

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