JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:47Generation of Recombinant Virus Particles
- 02:47Collection and Propagation of Virus Particles
- 05:11Viral Titer Evaluation
- 06:59Determining Replicative and Cytotoxic Capacities of Viral Vectors Encoding Immunomodulators
- 08:03Assessment of Immunological Functionality of Isolated Virus-encoded Immunomodulators from Infected Cells
- 10:00Results: Evaluation of Tumor-targeted Immunomodulation by Paramyxoviruses
- 11:37Conclusion
This protocol describes a detailed workflow for the generation and ex vivo characterization of oncolytic viruses for expression of immunomodulators, using measles viruses encoding bispecific T cell engagers as an example. Application and adaptation to other vector platforms and transgenes will accelerate the development of novel immunovirotherapeutics for clinical translation.
