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肿瘤靶向免疫调节副病毒的设计与评价

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JoVE 신문 암 연구학

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

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肿瘤靶向免疫调节副病毒的设计与评价

DOI:

10.3791/58651-v

•

12:42 min

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January 07, 2019

•

Johannes P.W. Heidbuechel², Christine E. Engeland³

¹Department of Translational Oncology,German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), ²Faculty of Biosciences,Heidelberg University, ³Department of Medical Oncology,NCT and Heidelberg University Hospital

Chapters

00:04Title
00:47Generation of Recombinant Virus Particles
02:47Collection and Propagation of Virus Particles
05:11Viral Titer Evaluation
06:59Determining Replicative and Cytotoxic Capacities of Viral Vectors Encoding Immunomodulators
08:03Assessment of Immunological Functionality of Isolated Virus-encoded Immunomodulators from Infected Cells
10:00Results: Evaluation of Tumor-targeted Immunomodulation by Paramyxoviruses
11:37Conclusion

Summary

자동 번역

该协议描述了一个详细的工作流程, 用于产生和体外表征的结肠病毒的免疫调节剂的表达, 使用麻疹病毒编码双特异性 t 细胞引擎为例。应用和适应其他载体平台和转基因将加速新的免疫病毒疗法的发展, 用于临床翻译。

Tags

Paramyxoviruses Tumor-targeted Immunomodulation Oncolytic Vectors Reverse Genetic System Measles Virus Bispecific T Cell Engager Virus Propagation Syncytia Formation Virus Rescue Transfection MV Producer Cells Reporter Gene Expression Virus Harvest

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