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1Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, 2Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, 3Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus
Chapters
- 00:04Title
- 00:45Reagents Preparation
- 02:01Plating Fibroblasts for Reprogramming: Day 0
- 05:09Initiation of Reprogramming: Day 1
- 09:28Results: Efficient Reprogramming of Human Primary Fibroblasts into iPSCs
- 11:16Conclusion
Her vi beskriver en klinisk relevant, høj effektivitet, feeder-fri metode til at omprogrammere menneskelige primære fibroblaster til induceret pluripotente stamceller ved hjælp af modificerede mRNAs kodning omprogrammering faktorer og modne mikroRNA-367/302 efterligner. Også inkluderet er metoder til at vurdere omprogrammering effektivitet, Udvid klonede iPSC kolonier, og bekræfte udtryk pluripotency mærkestof TRA-1-60.
