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면역학 및 감염병학

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猪全血表达编码和非编码 rna 类的鉴定

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

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猪全血表达编码和非编码 rna 类的鉴定

DOI:

10.3791/58689-v

•

09:40 min

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November 28, 2018

•

Damarius S. Fleming², Sarah J. Anderson, Laura C. Miller

¹Virus and Prion Research Unit, National Animal Disease Center,USDA, Agricultural Research Service, ²ORAU/ORISE

Chapters

00:04Title
00:48Processing of Swine Blood Samples and Organic Extraction for Total RNA and Small RNA
02:05Total RNA Isolation Procedure
03:33Globin Reduction
07:24Results: Expression Libraries of the Globin-depleted and Ribo-depleted Whole Blood Samples
08:50Conclusion

Summary

자동 번역

在这里, 我们提出了一个协议优化的处理编码 (mrna) 和非编码 (ncrna) 全球减少 rna-seq 库从一个单一的全血样本。

Tags

Keywords: RNA Classes Coding RNA Non-coding RNA Whole Blood Host-pathogen Interactions RNAseq Globin-reduced MRNA MiRNA Isolation Centrifugation Lysis Phenol-chloroform Ethanol Filter Cartridge Wash Solution

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