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면역학 및 감염병학

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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

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淋巴瘤组织学标本中多路荧光组织化学染色、成像与分析

DOI:

10.3791/58711-v

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07:52 min

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January 09, 2019

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Guo Hong*^1,2, Shuangyi Fan*³, The Phyu, Priyanka Maheshwari, Michal Marek Hoppe, Hoang Mai Phuong, Sanjay de Mel, Michelle Poon, Siok-Bian Ng³, Anand D. Jeyasekharan⁴

¹Department of Laboratory Medicine,AnSteel Group General Hospital, ²Cancer Science Institute of Singapore,National University of Singapore, ³Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, ⁴Department of Haematology-Oncology,National University Health System

Chapters

00:04Title
00:57Monoplex Tyramide-Based Immunofluorescence
03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
04:36Data Analysis
06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
07:10Conclusion

Summary

자동 번역

在这里, 我们描述了一个协议的多重荧光免疫组化染色和成像同时定位的多种癌症相关抗原在淋巴瘤。该协议可推广到所有组织切片中生物标志物的共化分析。

Tags

Keywords: Multiplexed Fluorescent Immunohistochemistry Lymphoma Tumor Microenvironment Antigen Co-expression Spatial Relationship Antigen Retrieval Microwave Stripping Peroxidase Block Tyramide-based Fluorescent Reagent Spectral Imaging

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