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Marquage immunohistochimique Fluorescent multiplexé, l’imagerie et l’analyse des échantillons histologique du lymphome
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1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System
Chapters
- 00:04Title
- 00:57Monoplex Tyramide-Based Immunofluorescence
- 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
- 04:36Data Analysis
- 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
- 07:10Conclusion
Nous décrivons ici un protocole pour multiplex immunohistochemical fluorescent de coloration et d’imagerie pour la localisation simultanée de plusieurs antigènes associés aux cancer des lymphomes. Ce protocole peut être étendu à l’analyse de colocalisation de biomarqueurs dans toutes les coupes de tissus.
