Journal
/
신경과학
/
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

DOI:
10.3791/58728-v

•

06:11 min

•

February 26, 2019

•

, ,
1Vollum Institute,Oregon Health and Science University, 2Harvard Medical School

Chapters

  • 00:04Conclusion
  • 00:35Mix Aqueous Silk with AAV Expression Vectors and Apply Silk/AAV Film to Devices
  • 04:11Results: Targeted Delivery of Expression Vectors Using Silk/AAV Optical Fibers
  • 05:24Conclusion

Summary

자동 번역

자동 번역

Here, we present a method for delivering viral expression vectors into the brain using silk fibroin films. This method allows targeted delivery of expression vectors using silk/AAV coated optical fibers, tapered optical fibers, and cranial windows.

Tags

OptogeneticsIn VivoTargeted ExpressionSilk/AAV FilmsNeuronal EnsemblesSensory ProcessingCognitive ProcessingChan RhodopsinGCaMPOptical ImplantsAAVSilk FibroinStereotaxic ApparatusMicroinjectorOptical FiberVacuum Desiccation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Read Article