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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
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Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

DOI:
10.3791/58748-v

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09:23 min

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November 28, 2018

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, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Chapters

  • 00:04Title
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

자동 번역

자동 번역

This article describes protocols to assess the effect of fluorescent proteins on the aggregation and toxicity of misfolded polyglutamine expansion for the rapid evaluation of a newly uncharacterized fluorescent protein in the context of fluorescent reporters.

Tags

Fluorescent ProteinsFusion PartnersPolyglutamine ToxicityYeastHTT Exon One25Q72QMonomeric Superfolder GFPGAL1 PromoterPolyQ FusionsOptical Density

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