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Analyse van Shear Flow-geïnduceerde migratie van RattenUitrustingen Marginal Zone B cellen In Vitro
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
DOI:

08:31 min

November 26, 2018

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Chapters

  • 00:04Title
  • 00:43Preparing the 6-chambered Slides for the Flow Experiment
  • 01:49Fluidics Unit Preparation
  • 03:07Cell Loading
  • 04:04Attaching the Fluidics Unit and Imaging the Cells
  • 05:28Migration Track Analysis
  • 07:02Results: Quantifying Marginal Zone B-cell Shuttling
  • 08:00Conclusion

Summary

자동 번역

Marginaal zone B cellen (MZBs) reageren op de kracht van shear stroom door heroriëntering op hun migratiepad van de stroom. Dit protocol laat zien hoe om te registreren en analyseren van het migratie met behulp van een fluidics eenheid, pomp, Microscoop imaging systeem en vrije software.

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