Journal
/
암 연구학
/
A Spheroid Killing Assay by CAR T Cells
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
A Spheroid Killing Assay by CAR T Cells

A Spheroid Killing Assay by CAR T Cells

DOI:
10.3791/58785-v

•

08:19 min

•

December 12, 2018

•

, , ,
1Department of Cellular Therapy, Department of Oncology,Oslo University Hospital-Radiumhospitalet

Chapters

  • 00:04Title
  • 00:31Colorectal Cancer Cell Line Spheroid Generation
  • 03:113D Tumor Spheroid Killing Assay
  • 05:00Automated Image Analysis
  • 06:59Results: Representative Spheroid Killing Analysis
  • 07:54Conclusion

Summary

자동 번역

자동 번역

This protocol is designed to assess immunotherapeutic redirected T-cell (CAR T-cell) cytotoxicity against 3D structured cancerous cells (spheroids) in real time.

Tags

3D Tumor CellsCAR T CellsCytotoxicity AssayColorectal CancerSpheroidAutomated ImagingTime-lapse MicroscopyImmunotherapy

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

A 3D Organotypic Melanoma Spheroid Skin Model

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Read Article