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用奇米蛋白构建功能蛋白区的鉴定

JoVE 신문
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Identification of Functional Protein Regions Through Chimeric Protein Construction

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用奇米蛋白构建功能蛋白区的鉴定

DOI:

10.3791/58786-v

•

11:39 min

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January 08, 2019

•

Juan M. Adrian-Segarra, Holger Lörchner², Thomas Braun², Jochen Pöling²

¹Department of Cardiac Development and Remodelling,Max Planck Institute for Heart and Lung Research, ²Partner site Rhein-Main,German Centre for Cardiovascular Research (DZHK)

Chapters

00:04Title
00:49Chimeric Protein Design
05:00Preparation for Molecular Cloning
07:05Polymerase Chain Reaction (PCR) Amplification of the Individual DNA Fragments Forming the Chimera and to Generate the Chimeric DNA Sequence
09:22Results: Generation of the Oncostatin M BC Loop Chimera
10:55Conclusion

Summary

자동 번역

结构上相关的蛋白质经常发挥不同的生物学功能。交换这些蛋白质的等效区域, 以创造嵌合蛋白是一种创新的方法, 以确定关键的蛋白质区域, 负责其功能分化。

Tags

Protein Chimera Functional Protein Regions Protein Structure Protein Sequence Protein Engineering Molecular Biology Structural Biology Protein Domain Exchange Protein Domain Swapping

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