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早产儿和足月婴儿脐带源性间充质干细胞的分离与鉴定
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JoVE 신문 발생학
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
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早产儿和足月婴儿脐带源性间充质干细胞的分离与鉴定

DOI:
10.3791/58806-v

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07:26 min

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January 26, 2019

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, , , , , , , , , , , , , , , ,
1Department of Pediatrics,Kobe University Graduate School of Medicine, 2Department of Pathology,Kobe Children’s Hospital, 3Department of Pediatrics,Hyogo College of Medicine, 4Department of Developmental Pediatrics,Shizuoka Children’s Hospital, 5Department of Pediatrics,Nihon University School of Medicine

Chapters

  • 00:04Title
  • 01:24Umbilical Cord Dissection
  • 03:08Isolation of Umbilical Cord Mesenchymal Stem Cells (UC-MSCs)
  • 04:16Culture of UC-MSCs
  • 05:42Results: Confirmation of Successful Isolation and Differentiation Capacity of UC-MSCs
  • 06:46Conclusion

Summary

자동 번역

자동 번역

人类脐带 (uc) 可以在围生期获得, 这是早产、足月和产后分娩的结果。在该协议中, 我们描述了在妊娠19-40周时从胎儿婴儿中分离和表征 uc 衍生的间充质干细胞 (uc-mscs) 的方法。

Tags

Keywords: Umbilical CordMesenchymal Stem CellsUCMSCPretermTerm InfantsCell TherapyIsolationCharacterizationDissectionEnzymatic DigestionCell CultureProliferation

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