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Chapters
- 00:04Title
- 00:38Dental Pulp Stem Cell (DPSC) Isolation and Culture
- 02:14DPSC Characterization and Differentiation
- 04:01Conditioned Medium Cancer Cell Treatment
- 05:09Cell Migration and Co-Culture Analyses
- 07:23Results: Representative Effects of Tumor Cell:DPSC Interactions
- 08:28Conclusion
We provide protocols for evaluation of mesenchymal stem cells isolated from dental pulp and prostate cancer cell interactions based on direct and indirect co-culture methods. Condition medium and trans-well membranes are suitable to analyze indirect paracrine activity. Seeding differentially stained cells together is an appropriate model for direct cell-cell interaction.
