Journal
/
유전학
/
Novel Sequence Discovery by Subtractive Genomics
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

DOI:
10.3791/58877-v

•

09:40 min

•

January 25, 2019

•

, ,
1Biology Department,American University

Chapters

  • 00:04Title
  • 01:08De novo Assemble Starting Sequence
  • 02:03BLAST the Assembly Against the Reference Sequence
  • 04:44Design Primers for the Sequence that Remains
  • 06:25qPCR Validation of the Remaining Sequence
  • 07:51Results: Example Data of qPCR Results Including Negative and Positive Outcomes
  • 08:53Conclusion

Summary

자동 번역

자동 번역

The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially known.

Tags

Keywords: Novel Sequence DiscoverySubtractive GenomicsGenomic SequencesInexpensiveFlexibleBacterial Vaccine TargetsVirus IdentificationUnknown SeparationReference SequenceTrimmomaticPearReptileTrinityBlastTransdeckorTblastn

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/58877?article_type=v

In Vitro Transcription Assays and Their Application in Drug Discovery
check_url/kr/58877?article_type=v

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
check_url/kr/58877?article_type=v

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
check_url/kr/58877?article_type=v

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
check_url/kr/58877?article_type=v

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
check_url/kr/58877?article_type=v

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
check_url/kr/58877?article_type=v

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
check_url/kr/58877?article_type=v

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
check_url/kr/58877?article_type=v

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
check_url/kr/58877?article_type=v

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Read Article