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Chapters
- 00:00Title
- 00:43Primary Myoblast Isolation
- 03:30Myoblast Proliferation and Differentiation
- 06:25Results: Representative Myoblast Nucleus Differentiation and Tracking Analyses
- 08:14Conclusion
Skeletal muscle differentiation is a highly dynamic process, which particularly relies on nuclear positioning. Here, we describe a method to track nuclei movements by live cell imaging during myoblast differentiation and myotube formation and to perform a quantitative characterization of nuclei dynamics by extracting information from automatic tracking.
