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1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics,Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory,Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics,Affiliated Hospital of Nanjing University of Chinese Medicine
Chapters
- 00:04TItle
- 00:55Traditional Method of Isolating Bone Marrow-derived Cells
- 02:40Improved Method of Isolating Bone Marrow-derived Cells
- 04:11Purification by Density Gradient Centrifugation
- 05:39Culture and Differentiation
- 06:15Bone Resorption Assay Using Toluidine Blue Staining
- 08:03Scanning Electron Microscopy
- 09:03Results: Analysis of the Osteoclast Precursors Isolated, Purified, and Differentiated from Rat Bone Marrow
- 10:40Conclusion
अस्थिकेलिस्ट्स ऊतक-विशिष्ट मैक्रोफेज पॉलीकार्यन्स होते हैं जो मोनोसाइट से व्युत्पन्न होते हैं-हेमेटोपोएटिक स्टेम कोशिकाओं की मैक्रोफेज वंशावली । यह प्रोटोकॉल अस्थि मज्जा की कोशिकाओं को अलग करने के तरीके का वर्णन करता है, ताकि पारंपरिक पद्धतियों में पाए जाने वाले दुर्घटनाओं के जोखिम को कम करते हुए ऑस्टियोक्लैस्ट्स की बड़ी मात्रा प्राप्त की जा सके ।
