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Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
 

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Article DOI: 10.3791/58931-v 06:32 min January 9th, 2019
January 9th, 2019

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요약

Neurospheres grown as 3D cultures constitute a powerful tool to study glioma biology. Here we present a protocol to perform immunohistochemistry while maintaining the 3D structure of glioma neurospheres through paraffin embedding. This method enables the characterization of glioma neurosphere properties such as stemness and neural differentiation.

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Keywords: 3D Glioma Neurosphere Cultures Immunohistochemistry Paraffin-embedded Biomarkers Tissue Processing Microtome Sectioning
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