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JoVE Journal Neuroscience
Un protocole en acier inoxydable pour l’ARN haute qualité laser capturer des cellules de Microdissected Purkinje du cervelet humain Post-Mortem
 
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Un protocole en acier inoxydable pour l’ARN haute qualité laser capturer des cellules de Microdissected Purkinje du cervelet humain Post-Mortem

Article DOI: 10.3791/58953-v • 09:04 min • January 17th, 2019
January 17th, 2019 •
1, 2,3,4, 1
1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

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Ce protocole utilise une approche sans tache de visualiser et d’isoler les cellules de Purkinje dans les tissus frais congelés de cervelet humain post-mortem par microdissection de saisie de laser. Ce protocole vise à produire des quantités suffisantes d’ARN de haute qualité pour RNA-sequencing.

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Numéro 143 cellule de Purkinje neuroscience cervelet saisie microdissection ARN RNAseq tremblement essentiel de laser
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