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用于全基因组 DNA 分析的超长读数测序

JoVE 신문
생체공학

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JoVE 신문 생체공학

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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用于全基因组 DNA 分析的超长读数测序

DOI:

10.3791/58954-v

•

10:34 min

•

March 15, 2019

•

Liang Gong, Chee-Hong Wong, Jennifer Idol, Chew Yee Ngan, Chia-Lin Wei

¹Genome Technologies,Jackson Laboratory for Genomic Medicine

Chapters

00:04Title
01:24High-molecular Weight (HMW) DNA Extraction
03:11Ultra-long DNA Library Construction – Mechanical Shearing-based Protocol
05:19Ultra-long DNA Library Construction – Transposase Fragmentation-based Protocol
06:23Sequencing on the Nanopore Device
07:52Results: Ultra-long DNA Sequencing Protocol
09:38Conclusion

Summary

자동 번역

长读序列极大地促进了复杂基因组的组合和结构变异的表征。我们描述了一种利用纳米粒子测序平台生成超长序列的方法。该方法采用优化的 DNA 提取, 然后进行改进的文库准备, 从人体细胞中产生数百条覆盖适度的千列酶读数。

Tags

Keywords: Ultra-long Read Sequencing Whole Genomic DNA Analysis High-molecular Weight DNA Repeat Expansion Disorders HLA-typing Precision Medicine DNA Extraction Mechanical Shearing Library Preparation

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