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基于细菌选择和生长的盘状细胞基因工程

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JoVE 신문 유전학

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

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基于细菌选择和生长的盘状细胞基因工程

DOI:

10.3791/58981-v

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06:08 min

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January 25, 2019

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Peggy Paschke, David A. Knecht, Thomas D. Williams, Peter A. Thomason, Robert H. Insall, Jonathan R. Chubb⁵, Robert R. Kay, Douwe M. Veltman

¹MRC Laboratory of Molecular Biology, ²Department of Molecular and Cell Biology,University of Connecticut, ³Cancer Research UK Beatson Institute Glasgow, ⁴MRC Laboratory for Molecular Cell Biology,University College London, ⁵Department of Cell and Developmental Biology,University College London

Chapters

00:04Title
00:54Preparation of Cells and Materials
02:30Transfection of Dictyostelium Cells Based on Bacterial Selectionin Protocol
04:15Results: Dual Reporter Extrachromosomal Plasmid System Demonstrated
05:37Conclusion

Summary

자동 번역

盘状双翅目是研究细胞迁移、内吞和发育等复杂细胞过程的常用模型生物。生物体的效用取决于基因操纵的可行性。在这里, 我们提出了方法, 以转移牙菌细胞, 克服现有的限制培养细胞在液体介质。

Tags

Dictyostelium Discoideum Genetic Engineering Bacterial Selection Growth On Bacteria Exochromosomal Plasmid Transfection Microscopy Klebsiella Aerogenes Sorensen Buffer LB Medium Optical Density H40 Electroporation Buffer SM Medium Agar Plate Cell Transfer Centrifugation DNA Transformation

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