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多孔硅薄膜作为神经生长因子载体的设计

JoVE 신문
생체공학

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JoVE 신문 생체공학

Designing Porous Silicon Films as Carriers of Nerve Growth Factor

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多孔硅薄膜作为神经生长因子载体的设计

DOI:

10.3791/58982-v

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10:12 min

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January 25, 2019

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Michal Rosenberg, Neta Zilony³, Orit Shefi³, Ester Segal⁴

¹Department of Biotechnology and Food Engineering,Technion – Israel Institute of Technology, ²Faculty of Engineering,Bar-Ilan University, ³Bar-Ilan Institute of Nanotechnologies and Advanced Materials, ⁴Russell Berrie Nanotechnology Institute,Technion – Israel Institute of Technology

Chapters

00:04Title
00:57Oxidized Porous Silicon (PSiO₂) Carrier Fabrication
02:10Nerve Growth Factor (NGF) Loading and Quantification and In Vitro NGF Release from PSiO₂ Carriers
04:42Cell Viability and Growth in the Presence of NGF-Loaded PSiO₂ Carriers
06:00Cell Differentiation Analysis
07:27Results: Representative Effects of NGF-Loaded PSiO₂ Carriers on Neuronal Growth and Development
08:58Conclusion

Summary

자동 번역

在这里, 我们提出了一个方案, 设计和制造纳米结构多孔硅 (psi) 薄膜作为可降解载体的神经生长因子 (ngf)。pc12 细胞和小鼠背根神经节 (drg) 神经元的神经分化和生长特征是在与 ngf 负载 psi 载体治疗时的特征。

Tags

Keywords: Porous Silicon Nerve Growth Factor NGF Carrier Bioactive Protein Reservoir Implant Fabrication Process Electrochemical Etching Thermal Oxidation Loading Solution ELISA

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