JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:45On-Chip Immobilization
- 02:22Collection of Animals from a Worm Sheet
- 02:53Application of Worm Sheets for Imaging Observations
- 03:11Treatment of Worm Sheets for Repeated Use
- 03:52Results: C. elegans Individuals Enclosed with PS Film Display No Reduction in Motility
- 04:20Conclusion
A series of immobilization methods has been established to allow the targeted irradiation of live Caenorhabditis elegans individuals using a recently developed ultra-thin polydimethylsiloxane microfluidic chip with water retention. This novel on-chip immobilization is also adequate for imaging observations. The detailed treatment and application examples of the chip are explained.
