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用于嵌合抗原受体 T 细胞的实时效力测定，用于定位固体和血液癌细胞

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

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用于嵌合抗原受体 T 细胞的实时效力测定，用于定位固体和血液癌细胞

DOI:

10.3791/59033-v

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08:46 min

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November 12, 2019

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Biao Xi, Robert Berahovich, Hua Zhou, Shirley Xu, Yuehua Wei, Jasper Guan, Hizkia Harto, Jian Guan, Lijun Wu, David Santa Ana³, Fabio Cerignoil, Brandon Lamarche, Yama A. Abassi, Vita Golubovskaya

¹ACEA Biosciences, Inc, ²ProMab Biotechnologies. Inc, ³ACEA Therapeutics

Chapters

00:04Title
00:30Chimeric Antigen Receptor (CAR)-Encoding Lentivirus Generation
02:29CAR T Cell Generation and Expansion
03:16CAR Expression Detection
04:55Real-Time Cytolysis Potency Assay
06:48Results: Representative CAR T Cell Target Cell Killing Efficacy as Assessed by RTCA
08:05Conclusion

Summary

자동 번역

我们描述了一个定量实时体外细胞解检测系统，以评估嵌合抗原受体T细胞针对液体和固体肿瘤细胞的效力。该协议可以扩展以评估其他免疫效应细胞，以及联合治疗。

Tags

Real-time Potency Assay Chimeric Antigen Receptor T Cells Solid And Hematological Cancer Cells CELLigence Real-time Cell Analysis Label-free Monitoring Target Cancer Cell Killing Effector Cell Types Combination Therapies Lentiviral Vector HEK293FT Cells Transfection Lentivirus Production Ultracentrifugation Quantitative RT-PCR CAR-T Cell Generation Peripheral Blood Mononuclear Cells CD3/CD28 Coated Microbeads

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