JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2Department of Soil, Water and Environmental Science,University of Arizona, 3Department of Earth Ocean and Atmospheric Sciences,Florida State University, 4Bruker Daltonics Inc., 5Biological Sciences Division,Pacific Northwest National Laboratory
Chapters
- 00:04Title
- 01:11Sequential Extraction of Organic Matter from Soil, Sediments, or Peat
- 02:42FTICR-MS Analysis
- 04:55Chemical Derivatization for GC-MS
- 06:14Liquid State NMR Analysis and LC-MS Lipidomics Analysis
- 07:22Proteomics Analysis
- 07:37Results: Number of Enzymes Identified in Minnesota Peat at Three Different Depths
- 09:04Conclusion
Este protocolo describe un rendimiento único para técnicas complementarias analíticas y ómicas que culmina en una caracterización totalmente emparejado de materia orgánica natural y proteómica microbiana en diferentes ecosistemas. Este enfoque permite comparaciones robustas para la identificación de rutas metabólicas y transformaciones importantes para describir la producción de gas de efecto invernadero y predecir las respuestas al cambio ambiental.
