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면역학 및 감염병학

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新生儿小鼠益生菌的研究

JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학

Probiotic Studies in Neonatal Mice Using Gavage

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新生儿小鼠益生菌的研究

DOI:

10.3791/59074-v

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10:36 min

•

January 27, 2019

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Freddy Francis, Natallia Varankovich, Byron Brook, Nelly Amenyogbe, Rym Ben-Othman, Bing Cai, Danny Harbeson, Aaron C. Liu, Ben Dai, Shelly McErlane, Kris Andrews, Tobias R. Kollmann*^1,2, Pinaki Panigrahi*³

¹Department of Experimental Medicine,University of British Columbia, ²Department of Pediatrics,University of British Columbia, ³Department of Epidemiology and Pediatrics,University of Nebraska Medical Centre, ⁴Animal Care Services,University of British Columbia

Chapters

00:00Title
00:04Intra-esophageal Gavage of Neonatal Mouse – Day of Life 2
05:19Collection of Intestinal Samples for Colonization Analysis
08:38Lactobacillus plantarum Colonization with Gavage Everyday or Every Other Day
09:03Lactobacillus plantarum Gavaged Mice Co-housed with Untreated Mice
09:28Conclusion

Summary

자동 번역

本研究详细介绍了将精确数量的益生菌灌胃给新生小鼠的过程。实验设置进行了优化, 包括但不限于益生菌给药、给药方法和肠道细菌的量化。

Tags

Probiotic Neonatal Mice Gavage Intestinal Microbiome Intra-esophageal Gavage Sterile Collection Representative Results Holding Nest Probiotic Dye Solution Feeding Needles Cross Contamination Air Bubbles

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