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JoVE Journal
Immunology and Infection

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Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice
 

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Article DOI: 10.3791/59088-v 11:20 min March 2nd, 2019
March 2nd, 2019

챕터

요약

Here we report common methods to analyze the phagocytic function of murine alveolar macrophages and bacterial clearance from the lung. These methods study in vitro phagocytosis of fluorescein isothiocyanate beads and in vivo phagocytosis of Pseudomonas aeruginosa Green Fluorescent Protein. We also describe a method for clearing P. aeruginosa in mice.

Tags

Keywords: Alveolar Macrophage Phagocytosis Pseudomonas Aeruginosa BSL-2 Intratracheal Delivery Bronchoalveolar Lavage BALF Primary Cell Isolation In Vitro Phagocytosis In Vivo Phagocytosis Bacterial Clearance
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