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1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York
Chapters
- 00:04Title
- 01:00Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
- 03:02Cell Lysis and Protein Transfer
- 05:28Histone Post-translational Modification Detection
- 06:52Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
- 08:03Conclusion
Dit protocol beschrijft experimentele procedures karakteriseren van genoom-brede veranderingen in de niveaus van Histon posttranslationele modificaties (PTM) die zich voordoen in verband met de overexpressie van eiwitten ALS en Parkinson in zijn gekoppeld Saccharomyces cerevisiae modellen. Na SDS-pagina de scheiding, worden individuele Histon PTM niveaus gedetecteerd met wijziging-specifieke antilichamen via het westelijke bevlekken.
